Selected article for: "master mix and Real time"
Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities
  • Document date: 2019_6_20
    • ID: u3pxycqh_25
    Snippet: Total cellular RNA was isolated using TRIzol reagent (Tiangen, Beijing, China). RT was performed using a Re-verTra Ace qPCR RT kit following the protocol of the manufacturer (Toyobo, Kyoto, Japan). Quantitative PCR was carried out utilizing SYBR green real-time PCR master mix (Toyobo). The RT and qPCR primers were described previously (41) ......
    Document: Total cellular RNA was isolated using TRIzol reagent (Tiangen, Beijing, China). RT was performed using a Re-verTra Ace qPCR RT kit following the protocol of the manufacturer (Toyobo, Kyoto, Japan). Quantitative PCR was carried out utilizing SYBR green real-time PCR master mix (Toyobo). The RT and qPCR primers were described previously (41) .

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