Author: ODEWUMI, CAROLINE; LATINWO, LEKAN M.; SINCLAIR, ANDRE; BADISA, VEERA L.D.; ABDULLAH, AHKINYALA; BADISA, RAMESH B.
Title: Effect of cadmium on the expression levels of interleukin-1a and interleukin-10 cytokines in human lung cells Document date: 2015_9_10
ID: roy4420g_7
Snippet: Crystal violet viability test. The viability test was performed as previously reported (25) . Briefly, to investigate the effect of CdCl 2 on the viability of the cells, ~1x10 5 A549 lung cancer cells or 5x10 4 normal MRC-9 lung cells were plated into each well of a 24-well tissue culture plate and allowed to stabilize overnight in a 5% CO 2 incubator at 37˚C. The cells were treated with 0, 25, 50, 75, 100, 125, 150 or 200 µM CdCl 2 in a final .....
Document: Crystal violet viability test. The viability test was performed as previously reported (25) . Briefly, to investigate the effect of CdCl 2 on the viability of the cells, ~1x10 5 A549 lung cancer cells or 5x10 4 normal MRC-9 lung cells were plated into each well of a 24-well tissue culture plate and allowed to stabilize overnight in a 5% CO 2 incubator at 37˚C. The cells were treated with 0, 25, 50, 75, 100, 125, 150 or 200 µM CdCl 2 in a final volume of 1 ml in triplicate wells and were subsequently incubated for 24 h at 37˚C in a 5% CO 2 incubator. Following incubation, the viability of the cells was measured. The median lethal dose (LD 50 ) value was calculated from the LD 50 graph where the two lines meet, according to a previous study (26) .
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