Author: Spence, Jennifer S.; Krause, Tyler B.; Mittler, Eva; Jangra, Rohit K.; Chandran, Kartik
Title: Direct Visualization of Ebola Virus Fusion Triggering in the Endocytic Pathway Document date: 2016_2_9
ID: tnaizwxo_33
Snippet: Expression of fluorescent endosome markers. For expression of fluorescently labeled endocytic pathway markers, cells were transduced with CellLight vectors (Life Technologies) for early endosomes (GFP-Rab5), late endosomes (GFP-Rab7), or lysosomes (GFP-LAMP1) 18 h prior to imaging according to the manufacturer's instructions. A retroviral packaging vector bearing NPC1-mNG was also generated, and cells were transduced 18 h in advance of imaging......
Document: Expression of fluorescent endosome markers. For expression of fluorescently labeled endocytic pathway markers, cells were transduced with CellLight vectors (Life Technologies) for early endosomes (GFP-Rab5), late endosomes (GFP-Rab7), or lysosomes (GFP-LAMP1) 18 h prior to imaging according to the manufacturer's instructions. A retroviral packaging vector bearing NPC1-mNG was also generated, and cells were transduced 18 h in advance of imaging.
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