Selected article for: "room temperature and TMB substrate"
Author: Jianghong An; Xuejiao Liao; Tongyang Xiao; Shen Qian; Jing Yuan; Haocheng Ye; Furong Qi; Chengguang Shen; Yang Liu; Lifei Wang; Xiaoya Cheng; Na Li; Qingxian Cai; Fang Wang; Jun Chen; Yingxia Liu; Yunfang Wang; Feng Zhang; Yang Fu; Xiaohua Tan; Lei Liu; Zheng Zhang
Title: Clinical characteristics of the recovered COVID-19 patients with re-detectable positive RNA test
  • Document date: 2020_3_30
    • ID: 58sc6xgq_15
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03. 26.20044222 doi: medRxiv preprint undetectable, the specimen was considered negative. All procedures involving clinical specimens and SARS-CoV-2 were performed in a biosafety level 3 laboratory. Meanwhile, we did next-generation sequencing of samples from three patients. antibodies solution (Sangon Biotech) were added to each plate, respecti.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.03. 26.20044222 doi: medRxiv preprint undetectable, the specimen was considered negative. All procedures involving clinical specimens and SARS-CoV-2 were performed in a biosafety level 3 laboratory. Meanwhile, we did next-generation sequencing of samples from three patients. antibodies solution (Sangon Biotech) were added to each plate, respectively, and incubated at 37°C for 60 minutes. After five washes, 100 μL of tetramethylbenzidine (TMB) substrate (Sangon Biotech) was added at room temperature in the dark. After 15 minutes, the reaction was stopped with a 2 M H2SO4 solution. The absorbance was measured at 450 nm. All samples were run in triplicate. The ELISA titers were determined by endpoint dilution.

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