Author: Zhou, Jie; Li, Cun; Sachs, Norman; Chiu, Man Chun; Wong, Bosco Ho-Yin; Chu, Hin; Poon, Vincent Kwok-Man; Wang, Dong; Zhao, Xiaoyu; Wen, Lei; Song, Wenjun; Yuan, Shuofeng; Wong, Kenneth Kak-Yuen; Chan, Jasper Fuk-Woo; To, Kelvin Kai-Wang; Chen, Honglin; Clevers, Hans; Yuen, Kwok-Yung
Title: Differentiated human airway organoids to assess infectivity of emerging influenza virus Document date: 2018_6_26
ID: z637eh2z_19
Snippet: PD of Human AOs. The AOs were split and maintained in AO medium for 3-7 d. The culture medium in half of the organoids was changed to PD medium, PneumaCult-ALI medium (STEMCELL Technologies) supplemented with 10 μM DAPT (Tocris). The organoids were then cultured in AO or PD medium for 16 d. Bright-field images were taken every 3 d. Diameters of individual organoids were measured with ImageJ (NIH). The movies of organoids were acquired using a to.....
Document: PD of Human AOs. The AOs were split and maintained in AO medium for 3-7 d. The culture medium in half of the organoids was changed to PD medium, PneumaCult-ALI medium (STEMCELL Technologies) supplemented with 10 μM DAPT (Tocris). The organoids were then cultured in AO or PD medium for 16 d. Bright-field images were taken every 3 d. Diameters of individual organoids were measured with ImageJ (NIH). The movies of organoids were acquired using a total internal reflection fluorescent (TIRF) microscope (Movie S1) and a Nikon Eclipse Ti2 Inverted Microscope System (Movies S2 and S3). At the indicated times, the organoids in the different media were harvested for detection of cellular gene expression or for flow cytometry analysis.
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