Author: Zhou, Jie; Li, Cun; Sachs, Norman; Chiu, Man Chun; Wong, Bosco Ho-Yin; Chu, Hin; Poon, Vincent Kwok-Man; Wang, Dong; Zhao, Xiaoyu; Wen, Lei; Song, Wenjun; Yuan, Shuofeng; Wong, Kenneth Kak-Yuen; Chan, Jasper Fuk-Woo; To, Kelvin Kai-Wang; Chen, Honglin; Clevers, Hans; Yuen, Kwok-Yung
Title: Differentiated human airway organoids to assess infectivity of emerging influenza virus Document date: 2018_6_26
ID: z637eh2z_19
Snippet: PD of Human AOs. The AOs were split and maintained in AO medium for 3-7 d. The culture medium in half of the organoids was changed to PD medium, PneumaCult-ALI medium (STEMCELL Technologies) supplemented with 10 μM DAPT (Tocris). The organoids were then cultured in AO or PD medium for 16 d. Bright-field images were taken every 3 d. Diameters of individual organoids were measured with ImageJ (NIH). The movies of organoids were acquired using a to.....
Document: PD of Human AOs. The AOs were split and maintained in AO medium for 3-7 d. The culture medium in half of the organoids was changed to PD medium, PneumaCult-ALI medium (STEMCELL Technologies) supplemented with 10 μM DAPT (Tocris). The organoids were then cultured in AO or PD medium for 16 d. Bright-field images were taken every 3 d. Diameters of individual organoids were measured with ImageJ (NIH). The movies of organoids were acquired using a total internal reflection fluorescent (TIRF) microscope (Movie S1) and a Nikon Eclipse Ti2 Inverted Microscope System (Movies S2 and S3). At the indicated times, the organoids in the different media were harvested for detection of cellular gene expression or for flow cytometry analysis.
Search related documents:
Co phrase search for related documents- AO medium and cellular gene expression: 1
- AO medium and culture medium: 1, 2
- AO medium and cytometry analysis: 1
- AO medium and flow cytometry analysis: 1
- AO medium and gene expression: 1
- bright field and culture medium: 1, 2
- cellular gene expression and gene expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- culture medium and different medium: 1, 2, 3, 4, 5, 6, 7, 8, 9
- culture medium and gene expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- culture medium and indicate time: 1
- cytometry analysis and flow cytometry analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cytometry analysis and gene expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- flow cytometry analysis and gene expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- gene expression and indicate time: 1, 2, 3
- gene expression and individual organoid: 1
Co phrase search for related documents, hyperlinks ordered by date