Dual role of chloroquine in liver ischemia reperfusion injury: reduction of liver damage in early phase, but aggravation in late phase

Fang, H; Liu, A; Dahmen, U; Dirsch, O

Animals. Male inbred Lewis rats, purchased from Charles River Laboratories (Sulzfeld, Germany), weighing within 250-320 g, were used in this study. All animals were housed under standard animal care conditions and had free access to water and rat chow ad libitum. All procedures were carried out according to the German Animal Welfare Legislation. Partial hepatic warm I/R. Partial hepatic warm I/R model was performed as described previously. 8 In brief, after opening the abdomen with a transversal incision, interlobular ligaments were dissected. A micro vascular clamp was used to interrupt the arterial and portal venous blood supply to the left lateral and median liver lobes for 60 or 90 min.