Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities Document date: 2019_6_20
ID: u3pxycqh_58
Snippet: We further examined whether the effect of GuHCl is specific to the NTPase and helicase-like activities of VP35 or due to some general denaturing effect. EBOV VP35 has been previous reported to antagonize interferon (IFN) response and be a viral suppressor of RNA interference (VSR) (30, 43, 44) . Our data show that GuHCl had little effect on IFN response in Huh7 cells with or without EBOV minigenome (Supplementary Figure S11B ) and did not affect .....
Document: We further examined whether the effect of GuHCl is specific to the NTPase and helicase-like activities of VP35 or due to some general denaturing effect. EBOV VP35 has been previous reported to antagonize interferon (IFN) response and be a viral suppressor of RNA interference (VSR) (30, 43, 44) . Our data show that GuHCl had little effect on IFN response in Huh7 cells with or without EBOV minigenome (Supplementary Figure S11B ) and did not affect the VSR activity of VP35 (Supplementary Figure S11C) . Moreover, the effect of GuHCl to Zika virus (ZIKV) NS3, which is a well-known monomeric RNA helicase (23) , and found that GuHCl affected neither the helicase activity of ZIKV NS3 in vitro nor the replication of ZIKV in 293T cells (Supplementary Figure S12) . These data confirm that the effects of GuHCl to EBOV VP35 and EBOV replicon are specific.
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