Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase Document date: 2016_8_19
ID: x5y551c8_10
Snippet: First, we used a siRNA (small interfering RNA) to reduce BECN1 expression in human embryonic kidney HEK293 cells, which are readily transfected and infected with E. chaffeensis. 54 E. chaffeensis proliferation was significantly reduced in BECN1 siRNA-transfected cells compared with cells transfected with the control scrambled siRNA (Fig. 3A) . Next, we treated infected THP-1 cells with a cell-permeable potent autophagy inhibitor, spautin-1. 58 Sp.....
Document: First, we used a siRNA (small interfering RNA) to reduce BECN1 expression in human embryonic kidney HEK293 cells, which are readily transfected and infected with E. chaffeensis. 54 E. chaffeensis proliferation was significantly reduced in BECN1 siRNA-transfected cells compared with cells transfected with the control scrambled siRNA (Fig. 3A) . Next, we treated infected THP-1 cells with a cell-permeable potent autophagy inhibitor, spautin-1. 58 Spautin-1 promotes the degradation of BECN1 by inhibiting 2 USPs (ubiquitin-specific peptidases), USP10 and USP13, which target BECN1. 58 Spautin-1 treatment of infected cells significantly decreased E. chaffeensis proliferation (Fig. 3B) . These results demonstrated that both class III PtdIns3K activation and BECN1 are required for E. chaffeensis replication, implying that autophagy induction by class III PtdIns3K complex is required for E. chaffeensis replication.
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