Author: ODEWUMI, CAROLINE; LATINWO, LEKAN M.; SINCLAIR, ANDRE; BADISA, VEERA L.D.; ABDULLAH, AHKINYALA; BADISA, RAMESH B.
Title: Effect of cadmium on the expression levels of interleukin-1a and interleukin-10 cytokines in human lung cells Document date: 2015_9_10
ID: roy4420g_6
Snippet: Maintenance of the cell lines. The human MRC-9 normal lung (cat. no. CCL-212) and human A549 lung cancer (cat. no. CCL-185) cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). The supplied frozen cells were cultured, according to the manufacturer's instructions. The cells were grown in 10 ml minimum essential medium (American Type Culture Collection) (MRC-9) or F12K (A549) medium, containing 100 U/ml penicilli.....
Document: Maintenance of the cell lines. The human MRC-9 normal lung (cat. no. CCL-212) and human A549 lung cancer (cat. no. CCL-185) cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). The supplied frozen cells were cultured, according to the manufacturer's instructions. The cells were grown in 10 ml minimum essential medium (American Type Culture Collection) (MRC-9) or F12K (A549) medium, containing 100 U/ml penicillin, 100 µg/ml streptomycin, 0.025 µg/ml amphotericin B and 10% FBS in T-75 cm 2 tissue culture flasks at 37˚C in a 5% CO 2 incubator (Nuaire Co., Plymouth, MN, USA).
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