Selected article for: "chain reaction and virus type"
Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities
  • Document date: 2019_6_20
    • ID: u3pxycqh_5
    Snippet: The construction of pFastBac HTB-MBP and pFast-Bac HTB-MBP-EBOV VP35, pFastBac HTB-MBP-Enterovirus type 71 (EV71) 2C, pFastBac HTB-MBP-Zika virus (ZIKV) NS3 have been described previously (38) . The cDNA fragment of EBOV VP35 (GenBank accession no.: AF086833.2) was amplified by polymerase chain reaction (PCR) from the plasmid containing full-length EBOV cDNA. The cDNA fragments of EBOV VP35, EV71 2C and ZIKV NS3 were cloned into the vector pFastB.....
    Document: The construction of pFastBac HTB-MBP and pFast-Bac HTB-MBP-EBOV VP35, pFastBac HTB-MBP-Enterovirus type 71 (EV71) 2C, pFastBac HTB-MBP-Zika virus (ZIKV) NS3 have been described previously (38) . The cDNA fragment of EBOV VP35 (GenBank accession no.: AF086833.2) was amplified by polymerase chain reaction (PCR) from the plasmid containing full-length EBOV cDNA. The cDNA fragments of EBOV VP35, EV71 2C and ZIKV NS3 were cloned into the vector pFastBac HTA or pFastBac HTB-MBP that was originated from pFastBac HTB (Invitrogen, Carlsbad, CA, USA), respectively. The mutations were generated as previously described (39) . The resulting plasmids were subjected to Bac-to-Bac baculovirus system to express the recombinant proteins with an MBP or His6 fused at the N-terminal. The primers used in this study are shown in Supplementary Table S1 .

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