Author: Fang, H; Liu, A; Dahmen, U; Dirsch, O
Title: Dual role of chloroquine in liver ischemia reperfusion injury: reduction of liver damage in early phase, but aggravation in late phase Document date: 2013_6_27
ID: qmut89kb_26
Snippet: Histopathology. Liver tissue was fixed in 4.5% buffered formalin and embedded in paraffin. Sections (4 mm) were cut and stained with hematoxylineosin. Histological evaluation was performed using a semiquantitative scoring system. 47 HMGB1 immunohistochemistry. HMGB1 immunohistochemical analysis was performed as described previously. 29 Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, sections were then incubated with.....
Document: Histopathology. Liver tissue was fixed in 4.5% buffered formalin and embedded in paraffin. Sections (4 mm) were cut and stained with hematoxylineosin. Histological evaluation was performed using a semiquantitative scoring system. 47 HMGB1 immunohistochemistry. HMGB1 immunohistochemical analysis was performed as described previously. 29 Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, sections were then incubated with HMGB1 antibody (1 : 500; Abcam, #ab18256, Cambridge, UK) for 1 h at room temperature. Detection was performed by using PowerVision goat-anti-Rabbit-AP (ImmunoLogic, DPVR-110AP, Duiven, the Netherlands) and using Fast-red (Abcam, #ab64254) as substrate.
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