Selected article for: "bovine serum and fetal bovine serum medium"
Author: Oh, Seo-ho; Kim Cho, Young-Saeng; Lee, Ho-Bin; Lee, Sang-Mok; Kim, Whee-Soo; Hong, Liang; Cho, Chong-Su; Choi, Yun-Jaie; Kang, Sang-Kee
Title: Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0
  • Document date: 2019_11_5
    • ID: q4p77ukw_15
    Snippet: Caco-2 cells (KCTC, Korea) were seeded at 1 × 10 5 per well in 48-well plates (SPL, Korea) and incubated at 37°C for 5 days in Dulbecco's modified Eagle's medium (Invitrogen, USA) supplemented with 10% fetal bovine serum (Gibco, USA) in an atmosphere containing 5% CO 2 . Cells were then cultivated overnight without FBS and subsequently stimulated with different concentrations of each recombinant protein for 6 h. The supernatants were harvested,.....
    Document: Caco-2 cells (KCTC, Korea) were seeded at 1 × 10 5 per well in 48-well plates (SPL, Korea) and incubated at 37°C for 5 days in Dulbecco's modified Eagle's medium (Invitrogen, USA) supplemented with 10% fetal bovine serum (Gibco, USA) in an atmosphere containing 5% CO 2 . Cells were then cultivated overnight without FBS and subsequently stimulated with different concentrations of each recombinant protein for 6 h. The supernatants were harvested, and cytokine levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits (Cusabio, China).

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