Selected article for: "acid extraction and adenovirus pcr"
Author: Shatizadeh, Somayeh; Yavarian, Jila; Rezaie, Farhad; Mahmoodi, Mahmood; Naseri, Maryam; Mokhtari Azad, Talat
Title: Epidemiological and clinical evaluation of children with respiratory virus infections
  • Document date: 2014_9_22
    • ID: yibdumij_11
    Snippet: Uniplex hemi nested PCR was done for detection of adenovirus. Briefly, 5 μL of the nucleic acid extraction was added to 45 μL of reaction mixture containing 10X PCR buffer, 4μL of Mgcl2 (50mM), 2.5μL of dNTP mix (10mM), 20 pmol of each degenerate primer pair and 0.4μL of taq polymerase. Temperature and time profiles were as follows: 1 cycle at 94° C for 3 min, followed by 30 cycles of 94° for 1 min, 50°C for 1 min, 68° C for 1 min, and f.....
    Document: Uniplex hemi nested PCR was done for detection of adenovirus. Briefly, 5 μL of the nucleic acid extraction was added to 45 μL of reaction mixture containing 10X PCR buffer, 4μL of Mgcl2 (50mM), 2.5μL of dNTP mix (10mM), 20 pmol of each degenerate primer pair and 0.4μL of taq polymerase. Temperature and time profiles were as follows: 1 cycle at 94° C for 3 min, followed by 30 cycles of 94° for 1 min, 50°C for 1 min, 68° C for 1 min, and final incubation at 68°C for 50 min. Then nested reaction was performed with 20 pmol of the another degenerate primer pair as above.

    Search related documents:
    Co phrase search for related documents
    • 10X PCR buffer and reaction mixture: 1
    • 10X PCR buffer and taq polymerase: 1, 2
    • degenerate primer pair and primer pair: 1, 2
    • dNTP mix and PCR buffer: 1, 2
    • dNTP mix and primer pair: 1
    • dNTP mix and reaction mixture: 1
    • dNTP mix and taq polymerase: 1, 2, 3
    • final incubation and time temperature: 1, 2
    • nucleic acid extraction and reaction mixture: 1, 2
    • nucleic acid extraction and taq polymerase: 1
    • PCR buffer and primer pair: 1, 2
    • PCR buffer and reaction mixture: 1, 2
    • PCR buffer and taq polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • primer pair and reaction mixture: 1
    • primer pair and taq polymerase: 1, 2, 3
    • primer pair and time temperature: 1
    • profile time temperature and time temperature: 1
    • reaction mixture and taq polymerase: 1, 2, 3, 4, 5
    • reaction mixture and time temperature: 1, 2