Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase Document date: 2016_8_19
ID: x5y551c8_48
Snippet: Colocalization analysis was performed with softWoRx software from DeltaVision microscope on a single z-section by counting 10 to 20 inclusions per cell in 5 to 20 cells per experiment from 3 independent experiments to obtain percentage colocalization of E. chaffeensis-inclusions with various markers including 2£FYVE, PIK3C3, GAPDH, and Etf-1. Colocalization of Etf-1 with RAB5, ATG5, or LC3 was analyzed by Coloc 2 in the NIH Image J software pack.....
Document: Colocalization analysis was performed with softWoRx software from DeltaVision microscope on a single z-section by counting 10 to 20 inclusions per cell in 5 to 20 cells per experiment from 3 independent experiments to obtain percentage colocalization of E. chaffeensis-inclusions with various markers including 2£FYVE, PIK3C3, GAPDH, and Etf-1. Colocalization of Etf-1 with RAB5, ATG5, or LC3 was analyzed by Coloc 2 in the NIH Image J software package for the Pearson correlation coefficient from 10 to 20 cells per group from 3 independent experiments. 126 Results were presented as average colocalization percentage or Pearson coefficients (r £ 100) § standard deviation, and data were analyzed by the Student unpaired t test for statistical significance.
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