Author: Lin, Huayuan; Huang, Qiqi; Guo, Xiaoquan; Liu, Ping; Liu, Weilian; Zou, Yuelong; Zhu, Shuliang; Deng, Guangfu; Kuang, Jun; Zhang, Caiying; Cao, Huabin; Hu, Guoliang
Title: Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers Document date: 2015_12_17
ID: t8547tse_11
Snippet: Total RNA was extracted from 12 individual chicken organs (12 kidneys) per group by homogenization in TranZol reagent (TransGen Biotech, China) using a homogenizer under low temperature according to the manufacturer's protocols. RNA was diluted in nuclease-free water, then quantified based on UV spectrophotometry at 260 nm. The integrity of the extracted RNA was confirmed by 0.8% agarose gel electrophoresis......
Document: Total RNA was extracted from 12 individual chicken organs (12 kidneys) per group by homogenization in TranZol reagent (TransGen Biotech, China) using a homogenizer under low temperature according to the manufacturer's protocols. RNA was diluted in nuclease-free water, then quantified based on UV spectrophotometry at 260 nm. The integrity of the extracted RNA was confirmed by 0.8% agarose gel electrophoresis.
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