Selected article for: "activity measure and luciferase assay kit"
Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities
  • Document date: 2019_6_20
    • ID: u3pxycqh_23
    Snippet: A BioLux Gaussia luciferase assay kit (New England Bio-Labs, Ipswich, MA, USA) was used to measure the GLuc activity. Culture supernatants were harvested and centrifuged at 10 000 g for 3 min. A 20-l volume of supernatant was added into a 96-well white plate, and 50 l of GLuc assay solution was added and mixed with culture supernatant immediately before measurement of the luminescence by the use of a VarioSkan Flash reader (Thermo Fisher Scientif.....
    Document: A BioLux Gaussia luciferase assay kit (New England Bio-Labs, Ipswich, MA, USA) was used to measure the GLuc activity. Culture supernatants were harvested and centrifuged at 10 000 g for 3 min. A 20-l volume of supernatant was added into a 96-well white plate, and 50 l of GLuc assay solution was added and mixed with culture supernatant immediately before measurement of the luminescence by the use of a VarioSkan Flash reader (Thermo Fisher Scientific).

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