Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities Document date: 2019_6_20
ID: u3pxycqh_27
Snippet: Cells were washed twice in cold PBS and lysed in lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP40, 0.25% deoxycholate and a protease inhibitor cocktail (Roche)], and the lysates were subjected to 10% SDS-PAGE and Western blotting according to our standard procedures (42) with the relevant antibodies. The anti-MBP and anti-â¤-actin antibodies were purchased from MBL Co. Ltd. The anti-NP, anti-VP30 and anti-VP35 antibodies were raised i.....
Document: Cells were washed twice in cold PBS and lysed in lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP40, 0.25% deoxycholate and a protease inhibitor cocktail (Roche)], and the lysates were subjected to 10% SDS-PAGE and Western blotting according to our standard procedures (42) with the relevant antibodies. The anti-MBP and anti-â¤-actin antibodies were purchased from MBL Co. Ltd. The anti-NP, anti-VP30 and anti-VP35 antibodies were raised in rabbits as previously described (41) .
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