Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities Document date: 2019_6_20
ID: u3pxycqh_11
Snippet: The recombinant baculovirus-infected Sf9 cells were resuspended, lysed by sonication and subject to centrifugation for 30 min at 11 000 g to remove debris. The protein in the supernatant was pulled down by using 5 -ATP agarose (Sigma-Aldrich) according to the manufacture's protocol. The purified ATP-bound protein was analyzed by western blotting with anti-MBP antibody. NTPase activities were determined via measuring the released inorganic phospha.....
Document: The recombinant baculovirus-infected Sf9 cells were resuspended, lysed by sonication and subject to centrifugation for 30 min at 11 000 g to remove debris. The protein in the supernatant was pulled down by using 5 -ATP agarose (Sigma-Aldrich) according to the manufacture's protocol. The purified ATP-bound protein was analyzed by western blotting with anti-MBP antibody. NTPase activities were determined via measuring the released inorganic phosphate during NTP hydrolysis using a direct colorimetric assay as previously described (21) .
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