Selected article for: "ASTRA software and chromatography software package"
Author: Shu, Ting; Gan, Tianyu; Bai, Peng; Wang, Xiaotong; Qian, Qi; Zhou, Hui; Cheng, Qi; Qiu, Yang; Yin, Lei; Zhong, Jin; Zhou, Xi
Title: Ebola virus VP35 has novel NTPase and helicase-like activities
  • Document date: 2019_6_20
    • ID: u3pxycqh_9
    Snippet: The affinity-purified protein sample was concentrated by tangential flow filtration using Amicon Ultra centrifugal filters (Merck) to 1 mg/ml for further analysis. For size exclusion chromatography, concentrated protein sample was mixed with BSA control and loaded onto a Superdex 200 increase 10/300 GL column (GE Healthcare) after pre-equilibration with buffer containing 50 mM HEPES-KOH (pH 8.0). Chromatography was taken with BioLogic Nucleic Aci.....
    Document: The affinity-purified protein sample was concentrated by tangential flow filtration using Amicon Ultra centrifugal filters (Merck) to 1 mg/ml for further analysis. For size exclusion chromatography, concentrated protein sample was mixed with BSA control and loaded onto a Superdex 200 increase 10/300 GL column (GE Healthcare) after pre-equilibration with buffer containing 50 mM HEPES-KOH (pH 8.0). Chromatography was taken with BioLogic Nucleic Acids Research, 2019, Vol. 47, No. 11 5839 DuoFlow system (Bio-Rad) at a flow rate of 1 ml/min. Peak analysis was performed using the ASTRA software package (BioLogic Chromatography Systems).

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