Author: Lee, Seung Heon; Yang, Dong-Kun; Kim, Ha-Hyun; Cho, In-Soo
Title: Efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs Document date: 2018_1_29
ID: w643jf90_10
Snippet: PEDV QIAP1401-p70 was chemically inactivated using binary ethyleneimine (BEI). In brief, 10 7.0 TCID50/mL virus were obtained from Vero cells cultured in roller bottles. After three rounds of freezing and thawing, the collected viral fluid was clarified and then inactivated by addition of 1 M BEI to a final concentration of 2 mM at 37°C overnight. The remaining BEI was neutralized by addition of 20% sodium thiosulfate. Virus inactivation was ver.....
Document: PEDV QIAP1401-p70 was chemically inactivated using binary ethyleneimine (BEI). In brief, 10 7.0 TCID50/mL virus were obtained from Vero cells cultured in roller bottles. After three rounds of freezing and thawing, the collected viral fluid was clarified and then inactivated by addition of 1 M BEI to a final concentration of 2 mM at 37°C overnight. The remaining BEI was neutralized by addition of 20% sodium thiosulfate. Virus inactivation was verified by the absence of viral growth in Vero cell cultures.
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