Author: Chen, Ai-Lan; Ou, Cai-Wen; He, Zhao-Chu; Liu, Qi-Cai; Dong, Qi; Chen, Min-Sheng
Title: Effect of hepatocyte growth factor and angiotensin II on rat cardiomyocyte hypertrophy Document date: 2012_10_15
ID: y9isrt3v_5
Snippet: Newborn Sprague-Dawley rats (18-20 g) were supplied by the Animal Center of Guangzhou Medical University. Rats were housed and used in accordance with our institutional guidelines for animal care and the Guide for Animal Care of the National Institutes of Health. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and TRIzol were purchased from Gibco BRL (USA). Trypsin was obtained from Amersco (USA). Alpha-sarcomeric actin was p.....
Document: Newborn Sprague-Dawley rats (18-20 g) were supplied by the Animal Center of Guangzhou Medical University. Rats were housed and used in accordance with our institutional guidelines for animal care and the Guide for Animal Care of the National Institutes of Health. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and TRIzol were purchased from Gibco BRL (USA). Trypsin was obtained from Amersco (USA). Alpha-sarcomeric actin was purchased from Santa Cruz Biotechnology (USA). The SYBR ® GreenPCR Master Mix was obtained from Applied Biosystems (USA). The Thermo ScriptRT-PCR kit was purchased from Invitrogen (USA). [ 3 H]-leucine (Leu) was obtained from the China Institute of Atomic Energy (China) and recombinant Ang II and HGF were purchased from Sigma (Germany).
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