Author: ZHANG, NAICHUN; DENG, JIANNING; WU, FENGYAO; LU, XIANGCHAN; HUANG, LEI; ZHAO, MIN
Title: Expression of arginase I and inducible nitric oxide synthase in the peripheral blood and lymph nodes of HIV-positive patients Document date: 2015_11_23
ID: zvnqiy9p_6
Snippet: Absolute CD4 + T cell count. The peripheral blood CD4 + T-cell counts were determined using the Multitest Imk Lymphocyte kit (cat. no. 340503; BD Biosciences, San Jose, CA, USA) and Trucount tubes (cat. no. 340504; BD Biosciences). 50 µl whole blood was collected and incubated with 10 µl antibodies for 20 min at room temperature. 1X BD Multitest IMK kit lysis solution was subsequently added to the tubes and after 15 min of incubation at room te.....
Document: Absolute CD4 + T cell count. The peripheral blood CD4 + T-cell counts were determined using the Multitest Imk Lymphocyte kit (cat. no. 340503; BD Biosciences, San Jose, CA, USA) and Trucount tubes (cat. no. 340504; BD Biosciences). 50 µl whole blood was collected and incubated with 10 µl antibodies for 20 min at room temperature. 1X BD Multitest IMK kit lysis solution was subsequently added to the tubes and after 15 min of incubation at room temperature, CD4 + T cells were counted using flow cytometry (FACSCanto II; BD Biosciences).
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