Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0

Oh, Seo-ho; Kim Cho, Young-Saeng; Lee, Ho-Bin; Lee, Sang-Mok; Kim, Whee-Soo; Hong, Liang; Cho, Chong-Su; Choi, Yun-Jaie; Kang, Sang-Kee

We also evaluated serum S0-specific IgG2a and IgG1 titers as indicators of Th1 and Th2 type immune responses [18] , respectively, at the 6th week postinjection. Data were expressed as a ratio of IgG2a/IgG1. Fig. 4F shows that the S0-immunized group had a more dominant S0-specific IgG1 response than IgG2a response, apart from one mouse. Mice from the S0-F or F-S0 immunized groups also had a more dominant S0-specific IgG1 response than IgG2a response. Interestingly, mice immunized with the S0/F mixture had a more dominant S0specific IgG2a response than IgG1 response (Fig. 4F) .