Selected article for: "mini kit and QIAcube platform"
Author: Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun
Title: Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples
  • Document date: 2014_4_8
    • ID: wci56l0c_13
    Snippet: The commercially available Seeplex DV mRT-PCR assay (Seegene, Seoul, Korea) was used to simultaneously detect group A rotaviruses, AdV 40 and 41 (species F), noroviruses GI and GII, and astroviruses [10, 11] . Nucleic acids were extracted from fecal suspensions by using QIAamp DNA Mini kit (Qiagen, Hilden, Germany) and QIAcube platform (Qiagen). The nucleic acid was amplified using a PTC-200 thermal cycler (Bio-Rad, Hercules, CA, USA), and the PC.....
    Document: The commercially available Seeplex DV mRT-PCR assay (Seegene, Seoul, Korea) was used to simultaneously detect group A rotaviruses, AdV 40 and 41 (species F), noroviruses GI and GII, and astroviruses [10, 11] . Nucleic acids were extracted from fecal suspensions by using QIAamp DNA Mini kit (Qiagen, Hilden, Germany) and QIAcube platform (Qiagen). The nucleic acid was amplified using a PTC-200 thermal cycler (Bio-Rad, Hercules, CA, USA), and the PCR products were visualized after electrophoresis on an agarose gel. All procedures were performed according to the manufacturers' instructions.

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