Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples

Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun

The prevalence rates of rotaviruses and adenoviruses detected by ICA were 20.3% and 7.5%, respectively. Our findings are slightly higher than those of a recent nationwide study that found 14% and 4.7% prevalence in the total population, and 2-5% and less than 1% in adults for rotaviruses and adenovirus, respectively [8] . However, our results were consistent with those reported in France (21.5% and 5%) [13] , but lower than those reported in China (41% and 12%) [14] , Japan (45% and 7.9%) [15, 16] , Canada (25.9% and 20.3%) [10] , and Ghana (55% and 28.2%) [17] , and slightly higher than the prevalence rates in Latin American countries (10-19% and 2%) [18] . The overall agreement rates among the 4 methods were 91.5% for rotaviruses, indicating comparable results among the 4 methods. The overall agreement, positive agreement, and negative agreement of ICA compared with mRT-PCR were 95.6%, 100%, and 94.9%, respectively. The discordant rate (4.4%) between the ICA and mRT-PCR was similar to that reported in previous studies (2.1-4.3%) [3, 9] . However, 17 stool samples showed positive results by using the ICA, but tested negative by using ELISA, ELFA, and mRT-PCR (Table 1) , and 15 of these 17 samples showed very weak positive band intensity by ICA. This finding suggests a high probability of false positive results by using ICA, especially when positive band intensity is very weak.