Selected article for: "comparative Ct method and Ct method"
Author: Narrandes, Shavira; Xu, Wayne
Title: Gene Expression Detection Assay for Cancer Clinical Use
  • Document date: 2018_6_5
    • ID: wheblwm3_8
    Snippet: The quantitation of results generally requires the study of the output dissociation curve, which allows a graph of known RNA concentration to be used as a reference for a curve constructed from the unknown RNA quantity. This approach is known as the standard curve method. The comparative Ct method involves comparing the Ct values of interest with a control, such as a non-treated or normal sample, which are both then normalized to a housekeeping g.....
    Document: The quantitation of results generally requires the study of the output dissociation curve, which allows a graph of known RNA concentration to be used as a reference for a curve constructed from the unknown RNA quantity. This approach is known as the standard curve method. The comparative Ct method involves comparing the Ct values of interest with a control, such as a non-treated or normal sample, which are both then normalized to a housekeeping gene. 9, 11 A biostatistical analysis can also be done where the final gene expression values are log-transformed for a normalized or symmetrical data distribution.

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