Author: Lee, Seung Heon; Yang, Dong-Kun; Kim, Ha-Hyun; Cho, In-Soo
Title: Efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs Document date: 2018_1_29
ID: w643jf90_15
Snippet: Serum-neutralizing antibody titers were determined by virus neutralization (VN) test in 96-well cell culture plates. Briefly, serum samples collected from the pigs after vaccination and challenge were diluted twofold (1:4 to 1:512) in serum-free DMEM containing 1 μg/mL trypsin and mixed with an equal volume of a virus suspension containing 100-200 TCID50/0.1 mL PEDV. After incubation for 1 hour at 37°C, 200 µL of the virus-serum mixture were a.....
Document: Serum-neutralizing antibody titers were determined by virus neutralization (VN) test in 96-well cell culture plates. Briefly, serum samples collected from the pigs after vaccination and challenge were diluted twofold (1:4 to 1:512) in serum-free DMEM containing 1 μg/mL trypsin and mixed with an equal volume of a virus suspension containing 100-200 TCID50/0.1 mL PEDV. After incubation for 1 hour at 37°C, 200 µL of the virus-serum mixture were added to confluent Vero cell monolayers and incubated for 1 hour at 37°C in 5% CO2. Thereafter, the cultures were washed three times with PBS containing 1 μg/mL trypsin and replenished with viral growth medium. After incubation for 48 hours, VN titers were established as reciprocal values of the highest serum dilution that inhibited PEDV-specific CPE.
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