Selected article for: "Mini kit and RNeasy Mini kit"
Author: Lee, Seung Heon; Yang, Dong-Kun; Kim, Ha-Hyun; Cho, In-Soo
Title: Efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs
  • Document date: 2018_1_29
    • ID: w643jf90_8
    Snippet: Viral RNA was extracted from 200 μL aliquots of QIAP1401-p70 using the RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The complete genome of the virus was sequenced at an external facility (Macrogen Inc., Seoul, Korea) by a next-generation sequencing technique. The whole genome sequence was compared with those of the following PEDV reference strains isolated in the United States: USA/Iowa303/2014 (GenBank.....
    Document: Viral RNA was extracted from 200 μL aliquots of QIAP1401-p70 using the RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The complete genome of the virus was sequenced at an external facility (Macrogen Inc., Seoul, Korea) by a next-generation sequencing technique. The whole genome sequence was compared with those of the following PEDV reference strains isolated in the United States: USA/Iowa303/2014 (GenBank No. KR265827), USA/Minnesota250/2014 (KR265776), and OH1414 (KJ408801). Multiple-sequence alignments were generated using Clustal X 2.0 [18] , and the results were visualized using ESPript 3.0 [19]

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