Author: Seong, Moon-Woo; Lee, Seung Jun; Cho, Sung Im; Ko, Kyungphil; Kim, Mi-Na; Sung, Heungsub; Kim, Jae-Seok; Ahn, Ji Soo; Yu, Byung Su; Kim, Taek Soo; Kim, Eui Chong; Park, Sung Sup
Title: External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak Document date: 2015_2_23
ID: uiou7c2b_20
Snippet: In the second phase EQA, panel results for 47 participants were 100% correct for all three samples tested ( Table 2 ). The difference in mean CT values between commercial kits in the second EQA phase was similar to that for the first EQA phase. The Pow-erChek kit showed the lowest CT values for upE and ORF1a, and these differences were statistically significant except for ORF1a in sample A (Sample A: P < 0.0001 and P = 0.0527 for upE and ORF1a, r.....
Document: In the second phase EQA, panel results for 47 participants were 100% correct for all three samples tested ( Table 2 ). The difference in mean CT values between commercial kits in the second EQA phase was similar to that for the first EQA phase. The Pow-erChek kit showed the lowest CT values for upE and ORF1a, and these differences were statistically significant except for ORF1a in sample A (Sample A: P < 0.0001 and P = 0.0527 for upE and ORF1a, respectively; sample B: P < 0.0001 and P = 0.0486 for upE and ORF1a, respectively). We analyzed the effect of real-time platform on the study results. Among the results obtained using the PowerChek kit, CT values from the ABI 7500 system were found to be lower than those from BioRad CFX96 in the two MERS-CoV-positive samples (Table 3 ). These differences were statistically significant except for ORF1a in sample B.
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