Selected article for: "chaperone tig and coli expression"
Author: Oh, Seo-ho; Kim Cho, Young-Saeng; Lee, Ho-Bin; Lee, Sang-Mok; Kim, Whee-Soo; Hong, Liang; Cho, Chong-Su; Choi, Yun-Jaie; Kang, Sang-Kee
Title: Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0
  • Document date: 2019_11_5
    • ID: q4p77ukw_26
    Snippet: In contrast to F-S0, which was expressed mostly as an insoluble protein, 59% of S0-F was expressed as a soluble protein. Trigger factor chaperone tig was used to enhance solubility, as it has been shown to assist protein folding by protecting long hydrophobic nascent chains during synthesis and initial folding stages [17] . We cotransformed the tig expression vector pTF16 (Fig. 2B) into E. coli BL21(DE3) together with each target protein's expres.....
    Document: In contrast to F-S0, which was expressed mostly as an insoluble protein, 59% of S0-F was expressed as a soluble protein. Trigger factor chaperone tig was used to enhance solubility, as it has been shown to assist protein folding by protecting long hydrophobic nascent chains during synthesis and initial folding stages [17] . We cotransformed the tig expression vector pTF16 (Fig. 2B) into E. coli BL21(DE3) together with each target protein's expression vector (pS0, pS0-F and pF-S0). Each target protein was induced by adding IPTG in the presence of tig, which had already been induced by L-arabinose addition. In this condition, 66% of S0 was expressed as a soluble protein, in contrast to S0-F and F-S0, which were almost entirely expressed as soluble proteins (Fig. 1B and D) . Interestingly, the total amount of F-S0 expression was 6.2 times higher than S0-F. Flagellin appeared to be expressed well in a soluble form without tig assistance. Each target protein in soluble and insoluble fractions was also confirmed by western blot analysis using anti-histag antibody (Supplementary Fig. 1 ). Data revealed that the soluble expression patterns among target proteins have similar tendency to those of Coomassie blue staining results in Fig. 2 .

    Search related documents:
    Co phrase search for related documents
    • blot analysis and expression pattern: 1, 2, 3, 4
    • blot analysis and expression vector: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • blot analysis and fold protein: 1, 2, 3, 4, 5, 6, 7, 8
    • blot analysis and insoluble protein: 1
    • blot analysis and insoluble soluble fraction: 1
    • chaperone tig and initial synthesis: 1
    • chaperone tig and insoluble protein: 1
    • expression pattern and fold protein: 1
    • expression pattern and fold stage: 1
    • expression vector and fold protein: 1, 2, 3
    • expression vector and insoluble protein: 1
    • fold protein and insoluble protein: 1, 2