Author: Gaikwad, Satish S.; Lee, Hyun-Jeong; Kim, Ji-Ye; Choi, Kang-Seuk
Title: Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein Document date: 2019_1_31
ID: tr3ageky_31
Snippet: NDV NP is known to be carry three antigenic regions, two of which are located at N terminal [5] . C terminal IDE (aa res-idues 444-459) ETQFLDLMRAVANSMR is a dispensable epitope on NP protein, which can be replaced with foreign epitope [4] . In preliminary study, when chickens were immunized with viable recombinant NDV lacking this IDE with reverse genetics technique, monomeric IDE peptide based ELISA showed poor sensitivity most likely due to sm.....
Document: NDV NP is known to be carry three antigenic regions, two of which are located at N terminal [5] . C terminal IDE (aa res-idues 444-459) ETQFLDLMRAVANSMR is a dispensable epitope on NP protein, which can be replaced with foreign epitope [4] . In preliminary study, when chickens were immunized with viable recombinant NDV lacking this IDE with reverse genetics technique, monomeric IDE peptide based ELISA showed poor sensitivity most likely due to small hapten size of 16 aa and immunosteric hindrance to bovine serum albumin conjugated peptide (data not shown). To address this issue, this problem can be solved by using multimers of IDE in the form of recombinant protein antigen.
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