Author: Gaikwad, Satish S.; Lee, Hyun-Jeong; Kim, Ji-Ye; Choi, Kang-Seuk
Title: Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein Document date: 2019_1_31
ID: tr3ageky_7
Snippet: Transformed cells were plated onto the Luria-Bertani agar containing kanamycin (50 μg/mL), gentamicin (7 μg/mL), tetracycline (10 μg/mL), Bluo-gal (100 μg/mL), and isopropylthio-β-galactoside (IPTG, 40 μg/mL) and incubated at 37°C for 36 to 48 hours. The high molecular weight bacmid DNA was isolated from the overnight cultures by alkaline lysis purification according to the manufacturer's manual of Bac-to-Bac baculovirus expression system .....
Document: Transformed cells were plated onto the Luria-Bertani agar containing kanamycin (50 μg/mL), gentamicin (7 μg/mL), tetracycline (10 μg/mL), Bluo-gal (100 μg/mL), and isopropylthio-β-galactoside (IPTG, 40 μg/mL) and incubated at 37°C for 36 to 48 hours. The high molecular weight bacmid DNA was isolated from the overnight cultures by alkaline lysis purification according to the manufacturer's manual of Bac-to-Bac baculovirus expression system (Invitrogen, Carlsbad, CA, USA).
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