Author: Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun
Title: Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples Document date: 2014_4_8
ID: wci56l0c_21
Snippet: Group A rotaviruses (ATCC VR-2018) were inoculated and cultured in MA104 cells. AdV40 (ATCC VR-931) and 41 (ATCC VR-930) were cultured in Graham 293 cells. Culture supernatants were 2-fold serially diluted with saline (1:2 to 1:1,024 dilutions) and used to evaluate the reproducibility and limit of detection of the ICA assay. Each diluted sample was tested in triplicate on each of 3 different lots of ICA for 10 days (Lot numbers: 113008, 113009, a.....
Document: Group A rotaviruses (ATCC VR-2018) were inoculated and cultured in MA104 cells. AdV40 (ATCC VR-931) and 41 (ATCC VR-930) were cultured in Graham 293 cells. Culture supernatants were 2-fold serially diluted with saline (1:2 to 1:1,024 dilutions) and used to evaluate the reproducibility and limit of detection of the ICA assay. Each diluted sample was tested in triplicate on each of 3 different lots of ICA for 10 days (Lot numbers: 113008, 113009, and 113010). The positive rates of repeated tests for each concentration were calculated for reproducibility.
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