Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples

Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun

The agreements between the ICA and other tests for the detection of rotavirus and adenovirus using clinical stool samples were assessed by positive agreement rate, negative agreement rate, total agreement rate, and kappa coefficient (к) (0.001-0. Table 3 ). The overall agreement among the 4 methods was 91.5% for rotaviruses. The positive rates of ICA, ELISA, real-time PCR, and mRT-PCR for adenovirus were 7.5%, 6.3%, 10.3%, and 3.5%, respectively ( Table 2 ). The positive agreement, negative agreement, and overall agreement rate of ICA compared with real-time PCR were 36.6%, 95.8%, and 89.8% (к = 0.368), respectively ( Table 3) . The positive agreement, negative agreement, and overall agreement rate of ICA compared with mRT-PCR were 71.4%, 94.8%, and 94.0% (к = 0.427), respectively. The overall agreement among the 4 methods was 85.5% for adenoviruses.