Selected article for: "MERS cov and neutralization test"
Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia
  • Document date: 2019_11_5
    • ID: vijh6x1l_9
    Snippet: Neutralization test using live MERS-CoV was performed as previously described except using Vero cells instead of Vero-TMPRSS2 cells [16] . Briefly, 0.05 ml of serially diluted test sera was mixed with an equal volume of 100 TCID 50 of MERS-CoV (EMC isolate) in a 96-well culture plate and incubated at 37°C for 1 hr; thereafter,Vero cells were added in each well and cultivated at 37°C. Cytopathic effects (CPE) on the Vero cells were observed at 3.....
    Document: Neutralization test using live MERS-CoV was performed as previously described except using Vero cells instead of Vero-TMPRSS2 cells [16] . Briefly, 0.05 ml of serially diluted test sera was mixed with an equal volume of 100 TCID 50 of MERS-CoV (EMC isolate) in a 96-well culture plate and incubated at 37°C for 1 hr; thereafter,Vero cells were added in each well and cultivated at 37°C. Cytopathic effects (CPE) on the Vero cells were observed at 3 days after infection. The neutralization titer was determined as the highest serum dilution showing at least 50% CPE on the inoculated cells.

    Search related documents:
    Co phrase search for related documents
    • CPE cytopathic effect and cytopathic effect: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77
    • CPE cytopathic effect and equal volume: 1, 2
    • CPE cytopathic effect and inoculated cell: 1, 2, 3, 4, 5
    • CPE cytopathic effect and Neutralization test: 1, 2, 3, 4, 5
    • CPE cytopathic effect and neutralization titer: 1
    • CPE cytopathic effect and serum dilution: 1, 2, 3
    • CPE cytopathic effect and test sera: 1, 2
    • CPE cytopathic effect and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • CPE cytopathic effect and Vero cell CPE cytopathic effect: 1, 2, 3, 4, 5, 6, 7
    • culture plate and cytopathic effect: 1, 2
    • culture plate and equal volume: 1
    • culture plate and Neutralization test: 1
    • culture plate and neutralization titer: 1
    • culture plate and serially diluted test sera: 1
    • culture plate and serum dilution: 1, 2
    • culture plate and test sera: 1
    • cytopathic effect and equal volume: 1, 2, 3, 4
    • cytopathic effect and inoculated cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • cytopathic effect and Neutralization test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12