Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia Document date: 2019_11_5
ID: vijh6x1l_9
Snippet: Neutralization test using live MERS-CoV was performed as previously described except using Vero cells instead of Vero-TMPRSS2 cells [16] . Briefly, 0.05 ml of serially diluted test sera was mixed with an equal volume of 100 TCID 50 of MERS-CoV (EMC isolate) in a 96-well culture plate and incubated at 37°C for 1 hr; thereafter,Vero cells were added in each well and cultivated at 37°C. Cytopathic effects (CPE) on the Vero cells were observed at 3.....
Document: Neutralization test using live MERS-CoV was performed as previously described except using Vero cells instead of Vero-TMPRSS2 cells [16] . Briefly, 0.05 ml of serially diluted test sera was mixed with an equal volume of 100 TCID 50 of MERS-CoV (EMC isolate) in a 96-well culture plate and incubated at 37°C for 1 hr; thereafter,Vero cells were added in each well and cultivated at 37°C. Cytopathic effects (CPE) on the Vero cells were observed at 3 days after infection. The neutralization titer was determined as the highest serum dilution showing at least 50% CPE on the inoculated cells.
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