Author: Gökçe, Sule; Kurugöl, Zafer; Koturoglu, Güldane; Çiçek, Candan; Aslan, Asli
Title: Etiology, Seasonality, and Clinical Features of Viral Respiratory Tract Infections in Children Hospitalized With Acute Bronchiolitis: A Single-Center Study Document date: 2017_6_22
ID: vbn98zua_8
Snippet: A nasal smear was obtained from each infant and tested for the presence of RSV, influenza virus types A and B, adenovirus, parainfluenza viruses, human rhinovirus, human coronavirus, human metapneumovirus, and human bocavirus with multiplex reverse-transcription PCR methods (RealAccurate, Respiratory RT PCR, PathoFinder, Netherlands, and Seeplex RV15 ACE Detection, Seegene, South Korea). Nasal samples were obtained more commonly by a nurse or som.....
Document: A nasal smear was obtained from each infant and tested for the presence of RSV, influenza virus types A and B, adenovirus, parainfluenza viruses, human rhinovirus, human coronavirus, human metapneumovirus, and human bocavirus with multiplex reverse-transcription PCR methods (RealAccurate, Respiratory RT PCR, PathoFinder, Netherlands, and Seeplex RV15 ACE Detection, Seegene, South Korea). Nasal samples were obtained more commonly by a nurse or sometimes a research assistant on all subjects within 48 hours of admission using a standardized protocol. 17 Samples were frozen at −20°C and transported on ice to the Department of Clinical Microbiology and Virology Laboratory of our university for viral nucleic acid amplification.
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