Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_48
Snippet: HeLa cells transfected with the indicated siRNAs were trypsinized 24 h after transfection. Cells (1 × 10 4 ) were plated in 24-well plates and incubated in complete growth medium. One day later, the cell number was measured every 24 h for 6 d using crystal violet staining, as described previously (Feinstein and Linstedt, 2007) . Briefly, cells were washed with PBS, fixed in 4% PFA for 30 min, stained with 0.5% crystal violet (in 30% methanol) fo.....
Document: HeLa cells transfected with the indicated siRNAs were trypsinized 24 h after transfection. Cells (1 × 10 4 ) were plated in 24-well plates and incubated in complete growth medium. One day later, the cell number was measured every 24 h for 6 d using crystal violet staining, as described previously (Feinstein and Linstedt, 2007) . Briefly, cells were washed with PBS, fixed in 4% PFA for 30 min, stained with 0.5% crystal violet (in 30% methanol) for 15 min, and extensively washed with H 2 O. The crystal violet was extracted using 1 ml of 10% acetic acid for 5 min, and the optical density was measured at 590 nm. The cell number at different times was normalized to the cell number from the first measurement (day 0).
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