Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_51
Snippet: For determination of total protein synthesis, 40 µl of the cell lysate was precipitated with 10 µl of 100% (wt/vol) TCA at 4°C for 10 min. The protein pellets were washed with acetone, dissolved in 0.2 M NaOH, neutralized with 0.2 M HCl, and mixed with Scintiverse BD cocktail (Fisher Scientific, cat. no. B14001). The incorporation of radioactivity was analyzed using scintillation counting and normalized based on the total protein amount......
Document: For determination of total protein synthesis, 40 µl of the cell lysate was precipitated with 10 µl of 100% (wt/vol) TCA at 4°C for 10 min. The protein pellets were washed with acetone, dissolved in 0.2 M NaOH, neutralized with 0.2 M HCl, and mixed with Scintiverse BD cocktail (Fisher Scientific, cat. no. B14001). The incorporation of radioactivity was analyzed using scintillation counting and normalized based on the total protein amount.
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