Author: Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun
Title: Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples Document date: 2014_4_8
ID: wci56l0c_5
Snippet: Four hundred stool samples were collected from patients with symptoms of acute gastroenteritis, from 6 university hospitals and 1 commercial laboratory, between October 2011 and March 2012. Patient ages ranged from 1 week to 77 yr (average, 17.4 yr); 233 samples (58.3%) were from patients under 5 yr of age. The SD Rota/Adeno Rapid test was performed immediately on the fresh stool samples. Each stool sample was diluted to a 10% stool suspension in.....
Document: Four hundred stool samples were collected from patients with symptoms of acute gastroenteritis, from 6 university hospitals and 1 commercial laboratory, between October 2011 and March 2012. Patient ages ranged from 1 week to 77 yr (average, 17.4 yr); 233 samples (58.3%) were from patients under 5 yr of age. The SD Rota/Adeno Rapid test was performed immediately on the fresh stool samples. Each stool sample was diluted to a 10% stool suspension in phosphate-buffered saline and stored at -70°C until they were used for the comparative tests (ELISA, ELFA, real-time PCR, and mRT-PCR assay). This study was approved by the Institutional Review Board of Hangang Sacred Heart Hospital, Hallym University College of Medicine (IRB No. 2011-217).
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