Selected article for: "bovine serum and FBS fetal bovine serum"

Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia
  • Document date: 2019_11_5
  • ID: vijh6x1l_2
    Snippet: Cell culture 293T and Vero cells were cultivated in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum (FBS), 100 U/ml of penicillin, and 100 µg/ml of streptomycin at 37°C [8] . 293T cells were used to prepare VSV-MERS/GFP and MERS-CoV recombinant receptor binding domain (RBD) for the cELISA antigen. Vero cells were used for neutralization tests by live MERS-CoV and VSV-MERS/GFP, respectively......
    Document: Cell culture 293T and Vero cells were cultivated in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum (FBS), 100 U/ml of penicillin, and 100 µg/ml of streptomycin at 37°C [8] . 293T cells were used to prepare VSV-MERS/GFP and MERS-CoV recombinant receptor binding domain (RBD) for the cELISA antigen. Vero cells were used for neutralization tests by live MERS-CoV and VSV-MERS/GFP, respectively.

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