Selected article for: "neutralization test and VSV MERS gfp"
Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia
  • Document date: 2019_11_5
    • ID: vijh6x1l_22
    Snippet: A comparison of the two neutralization tests using VSV-MERS/GFP and MERS-CoV showed a high correlation between both antibody titers. The former showed an antibody titer of ≥256, whereas the latter showed an antibody titer of ≥512. S1-ELISA was not sensitive compared to other tests because only 16 serum samples were positive and they required an antibody titer of ≥64 in VSV-MERS/GFP. However, the sensitivity of S1-ELISA would be improved by .....
    Document: A comparison of the two neutralization tests using VSV-MERS/GFP and MERS-CoV showed a high correlation between both antibody titers. The former showed an antibody titer of ≥256, whereas the latter showed an antibody titer of ≥512. S1-ELISA was not sensitive compared to other tests because only 16 serum samples were positive and they required an antibody titer of ≥64 in VSV-MERS/GFP. However, the sensitivity of S1-ELISA would be improved by examining the cut-off value and dilution of the test serum. In cELISA, five serum samples were negative out of the 31 positive samples in the neutralization tests, and an antibody titer of 16 was observed in both neutralization tests. Therefore, neutralization test using VSV-MERS/GFP exhibits sensitivity similar to the neutralization test using MERS-CoV and is more sensitive compared with the S1-ELISA as well as cELISA.

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