Author: Li, Hai; Wang, Fengjie; Han, Zongxi; Gao, Qi; Li, Huixin; Shao, Yuhao; Sun, Nana; Liu, Shengwang
Title: Genome-Wide Gene Expression Analysis Identifies the Proto-oncogene Tyrosine-Protein Kinase Src as a Crucial Virulence Determinant of Infectious Laryngotracheitis Virus in Chicken Cells Document date: 2015_12_17
ID: qwrdr92h_10
Snippet: Plaque assay. Liquid medium-based plaque assays were performed as previously described (39) , with minor modifications. Briefly, LMH cells were exposed to virus that was diluted in serum-free DMEM for 3 h at 37°C in 5% CO 2 and then washed with phosphate-buffered saline (PBS). The cells were subsequently covered with DMEM containing 10% FBS, fixed with 70% ethanol at each time point postinfection indicated below, and stained with 0.1% crystal vi.....
Document: Plaque assay. Liquid medium-based plaque assays were performed as previously described (39) , with minor modifications. Briefly, LMH cells were exposed to virus that was diluted in serum-free DMEM for 3 h at 37°C in 5% CO 2 and then washed with phosphate-buffered saline (PBS). The cells were subsequently covered with DMEM containing 10% FBS, fixed with 70% ethanol at each time point postinfection indicated below, and stained with 0.1% crystal violet.
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