Selected article for: "negative test and previously negative positive test"
Author: Gaikwad, Satish S.; Lee, Hyun-Jeong; Kim, Ji-Ye; Choi, Kang-Seuk
Title: Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein
  • Document date: 2019_1_31
    • ID: tr3ageky_15
    Snippet: Optimal dilutions of rERP and sera were determined by a checkerboard titration test with NDV positive and negative sera previously confirmed by hemagglutionation inhibition (HI) test. The antigen was coated in 96-well ELISA plates (PolySorb, Nunc, Roskilde, Denmark) ranging in concentration from 2 μg/mL to 0.0625 μg/mL in 50 mM carbonate/bicarbonate buffer (pH 9.6). Reference positive and negative sera were both diluted serially from 1:100-1:6,.....
    Document: Optimal dilutions of rERP and sera were determined by a checkerboard titration test with NDV positive and negative sera previously confirmed by hemagglutionation inhibition (HI) test. The antigen was coated in 96-well ELISA plates (PolySorb, Nunc, Roskilde, Denmark) ranging in concentration from 2 μg/mL to 0.0625 μg/mL in 50 mM carbonate/bicarbonate buffer (pH 9.6). Reference positive and negative sera were both diluted serially from 1:100-1:6,400 and tested to determine the optimal serum dilution. The dilutions that gave the optimum difference in absorbance at 450 nm between posi-tive and negative sera were selected to test the sera panel. The working dilution of goat anti-chicken HRP-IgG (Sigma-Aldrich), the reaction temperature, time and other conditions also were optimized.

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