Follow-up monitoring in a cat with leishmaniosis and coinfections with Hepatozoon felis and ‘Candidatus Mycoplasma haemominutum’

Attipa, Charalampos; Neofytou, Kyriaki; Yiapanis, Christos; Martínez-Orellana, Pamela; Baneth, Gad; Nachum-Biala, Yaarit; Brooks-Brownlie, Harriet; Solano-Gallego, Laia; Tasker, Séverine

Treatment with allopurinol at 10 mg/kg PO q12h was started (month 0) and administered for 6 months. The cat was examined every 2 months during treatment and then 8 months post-treatment cessation (month 14), with blood samples collected each time for haematology and biochemistry analysis. An ELISA was used to monitor L infantum-specific antibodies in serum, 6 as well as qPCR 4 for calculating the L infantum DNA levels in the blood as previously described (Table 1) . 7 The L infantum DNA level of the surgically excised skin nodule was high (approximately 3.48 × 10 10 relative copy numbers). Additionally, PCR assays were repeated for CMhm and Hepatozoon species.