Genome-Wide Gene Expression Analysis Identifies the Proto-oncogene Tyrosine-Protein Kinase Src as a Crucial Virulence Determinant of Infectious Laryngotracheitis Virus in Chicken Cells

Li, Hai; Wang, Fengjie; Han, Zongxi; Gao, Qi; Li, Huixin; Shao, Yuhao; Sun, Nana; Liu, Shengwang

Virus strain and cell line. The LJS09 strain of ILTV (GenBank accession no. JX458822) (15) is a virulent strain of ILTV that was isolated in China, and its genome has been sequenced. It was isolated in 2009 from 6-week-old Yellow-Foot layers housed on a farm in Jiangsu Province, China, which had not been vaccinated against ILTV, and it was stored at the Harbin Veterinary Research Institute of the CAAS. This strain can be propagated in a chemically immortalized LMH cell line, in which clear cytopathic effects (CPEs) have been observed (37, 38) . LMH cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, 100 g/ml streptomycin, and 2 mM L-glutamine. Cell cultures were incubated at 37°C in 5% CO 2 . The Src inhibitor SU6656 and the FAK inhibitor PF573228 were used at 1 and 10 M, respectively, for in vitro experiments. Control groups were treated with DMSO at the same concentrations.