Genome-Wide Gene Expression Analysis Identifies the Proto-oncogene Tyrosine-Protein Kinase Src as a Crucial Virulence Determinant of Infectious Laryngotracheitis Virus in Chicken Cells

Li, Hai; Wang, Fengjie; Han, Zongxi; Gao, Qi; Li, Huixin; Shao, Yuhao; Sun, Nana; Liu, Shengwang

To explore the effect of virus dosage on the virulence and replication of ILTV in ovo, 9-day-old SPF chicken embryos were inoculated with high (1 Ï« 10 4 TCID 50 ) or low (1 Ï« 10 3 TCID 50 ) doses of ILTV with or without the administration of 100 g of SU6656. All embryos inoculated with the high viral dose in the presence of 100 g of SU6656 were dead by the end of the observation period, while no embryo deaths occurred in any other group (Fig. 5A) . SU6656-mediated reductions of viral replication were observed in the allantoic fluids (Fig. 5B) , CAMs (Fig. 5C) , and livers (Fig. 5D ) of embryos inoculated with either dose of ILTV, which rules out the possibility that SU6656 did not affect viral replication in embryos inoculated with a low dose of virus, thereby demonstrating that the difference in survival rates between the embryos inoculated with high or low doses of ILTV (in combination with 100 g of SU6656) resulted from the different sizes of the viral inoculum.