Author: Yoo, Hyun Jung; Yoon, Sung Soo; Park, Seon Yang; Lee, Eun Young; Lee, Eun Bong; Kim, Ju Han; Song, Yeong Wook
Title: Gene Expression Profile during Chondrogenesis in Human Bone Marrow derived Mesenchymal Stem Cells using a cDNA Microarray Document date: 2011_6_20
ID: unr44dvp_13
Snippet: Total RNA was extracted from undifferentiated MSCs and from pellets after 3, 7, 14, or 21 days of differentiation using RNeasy kits (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. And 1,200 μL of RLT buffer supplemented with beta-mercaptoethanol (10 μL/mL) was added to the washed cells. RNA integrity was assessed by gel electrophoresis and RT-PCR and concentrations were determined by measuring absorbance at 260 nm......
Document: Total RNA was extracted from undifferentiated MSCs and from pellets after 3, 7, 14, or 21 days of differentiation using RNeasy kits (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. And 1,200 μL of RLT buffer supplemented with beta-mercaptoethanol (10 μL/mL) was added to the washed cells. RNA integrity was assessed by gel electrophoresis and RT-PCR and concentrations were determined by measuring absorbance at 260 nm.
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