Author: Narrandes, Shavira; Xu, Wayne
Title: Gene Expression Detection Assay for Cancer Clinical Use Document date: 2018_6_5
ID: wheblwm3_17
Snippet: Normalization is required to remove the variation in an experiment that affects gene expression levels and is necessary in the earlier stages of microarray examination as the following analyses will be affected by the normalization. Following imaging of the microarray, the raw data is first analyzed by subtracting the background fluorescence from the fluorescence of each spot. Selecting an appropriate housekeeping gene or genes is a delicate task.....
Document: Normalization is required to remove the variation in an experiment that affects gene expression levels and is necessary in the earlier stages of microarray examination as the following analyses will be affected by the normalization. Following imaging of the microarray, the raw data is first analyzed by subtracting the background fluorescence from the fluorescence of each spot. Selecting an appropriate housekeeping gene or genes is a delicate task as some genes previously thought to be ideal references have been shown to vary in expression level, depending on circumstances such as a donor's biological state. 28 A common normalization method, such as the non-linear locally weighted scatterplot smoothing (LOWESS) method, is applied to the background-adjusted intensities. 29
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